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Related post: GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00280-02 PERIOD COVERED October 1, 1982 to September 30, 1983 TITLE OF PROJECT (80 characteri or leas. Title must fit on one line between the borden.) Ecotropic murine leukemia virus proviral sequences in inbred mice, PRINCIPAL INVESTIGATOR (List other professional personnel on subsequent pages.) (Name, title, Purchase Serevent Online laboratory, and institute affiliation) Charles E. Buckler, Research Bioloaist, LVD, NIAID COOPERATING UNITS (if any) M. A. Order Serevent Martin, LMM, NIAID LAB/BRANCH Laboratory of Viral Diseases SECTION Viral Oncology Section NSTITUTE AND LOCATION National Institute of Allergy and Infectious Diseases TOTAL MANYEARS: .25 PROFESSIONAL: .25 CHECK APPROPRIATE BOX(ES) I I (a) Human subjects □ (b) Human tissues Ixl (c) Neither □ (a1) Minors □ (a2) Interviews SUMMARY OF WORK (Use standard unreduced type. Do not exceed the space provided.) The Fv-4 gene of mice confers resistance to infection by MuLV, This dominant locus which maps to chromosome 12 has been found to be associated Order Serevent Online with DNA sequences that hybridize to an ecotropic MuLV env- specific probe. A physical map of the oortion of the mouse genome that contains the Fv-4 related ecotropic env Generic Serevent sequences has been determined. The relative locations of 12 restriction sites in a reaion of 23 kilobases of mouse genome that surround the Fv-4 MuLV env related Buy Serevent Online sequence were obtained. The restriction map is unlike that of any known MuLV and thus the Fv-4 locus represents either a novel provirus or a fragment of a MuLV orovirus containing only env related sequences. The physical map obtained will aid in the design of experiments to clone the segment of the mouse genome that contains the Fv-4 locus. 26-10 PHS 6040 (Rev. 1/83) GPO 895-100 DEPARTMENT OF HEALTH AND HUMAN Buy Cheap Serevent SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00281-02 LVD PERIOD COVERED October 1, 1982 to September 30, 1983 TITLE OF PROJECT (80 characters or lees. Title must fit on one line between Purchase Serevent the borders.) Molecular cloning of recombinant MuLV proviral DNA sequences from AKR tumors PRINCIPAL INVESTIGATOR (List other professional personnel on subsequent pages.) (Name, title, laboratory, and institute affiliation) Charles E. Buckler, Research Biologist, LVD, NIAID COOPERATING UNITS (if any) T. Theodore and M.A. Martin, LMM, NIAID LAB/BRANCH Laboratory of Viral Diseases SECTION Viral Oncology Section INSTITUTE AND LOCATION National Institute of Allergy and Infectious Diseases TOTAL MANYEARS: .75 PROFESSIONAL: .75 CHECK APPROPRIATE BOX(ES) □ (a) Human subjects □ (a1) Minors □ (a2) Interviews □ (b) Human tissues [5] (c) Neither SUMMARY OF WORK (Ose standard unreduced type. Do not exceed the space provided.) The molecular cloning of recombinant MuLV proviral sequences present in thymomas of AKR mice is in progress, DNAs prepared from thymomas induced by the inocula- tion of 19 AKR/J neonates with MCF-247 viruses were analyzed by Southern blot hybridization. The tumors contain multiple copies of recombinant MuLV provirus DNA sequences in addition to the endogenous Buy Serevent MuLV-related sequences present in the AKR/J genome. A DNA library has been constructed from one tumor and a recombinant proviral sequence has been isolated from the library. Molecular subclones from the isolated recombinant containing flanking mouse cellular DNA sequences will be utilized to examine insertion sites in other tumors. Addi- tional libraries from other tumors will also be constructed to evaluate whether common integration regions play a role in the pathology of MuLV induced tumors . 26-11 PHS 6040 (Rev. 1/83) GPO 895-100 DCPARTMENT OF HEALTH AND HUMAN SERVICES- PUBLIC HFALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00282-01 LVD PERIOD COVERED October 1, 1982 to September 30, 1983 TITLE OF PROJECT (80 charactert or IcM. Title mU8l fit on one line between the borden.)
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